Vapro® Vapor Pressure Osmometer - Discovery Diagnostics, Canadian distributor for Wescor's Vapro Vapour Pressure Osmometers
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Vapro® Vapor Pressure Osmometer

Discovery Diagnostics, Canadian distributor for Wescor's Vapro® Vapor Pressure Osmometer for osmotic measurement of whole blood, viscous samples & tissue specimens


Model 5520 Vapro® Vapor Pressure Osmometer. A high-precision vapor pressure osmometer that produces accurate osmolality values on 10 microliters of a wide range of solutions from biological and industrial sources, especially valuable in research. The most advanced, easy to use vapor pressure osmometer ever developed.


Hemotology Slide Stainer Cytocentrifuge Product Details
The menu-driven Vapro® Osmometer determines osmolality at room temperature with sample in natural equilibrium.

What you don't get are cryoscopic artifacts due to high viscosity, suspended particles, or other conditions that interfere with freezing point determinations. The superbly accurate Vapor Pressure Osmometer has a much broader range of error-free applications.

Functions are selected from a simple menu. The Vapro® Vapor Pressure Osmometer is self-calibrating and exceptionally stable. Built in self-diagnostics alert you to any malfunction or procedural error. Statistical functions display average and standard deviation of several samples, and the handy clean test monitors thermocouple cleanliness. Regardless of the application, the Vapro® Osmometer will be a remarkable partner in the laboratory.


In most solutions, changes in concentration are accompanied by linear and proportional changes in the cardinal colligative properties of the solvent-vapor pressure, freezing point and boiling point. Measuring any of these properties provides an indirect indication of osmolality, but among them, only vapor pressure can be determined without a forced change in the sample's physical state.

In the Vapro osmometer, vapor pressure is determined thermometrically by a fine wire thermocouple suspended in a small vapor space above the specimen in a sealed sample chamber. During a measurement cycle, the thermocouple undergoes a series of microprocessor controlled temperature changes.

Step 1

After sample insertion, temperature and vapor pressure naturally equilibrate in the sealed chamber. The thermocouple senses the exact temperature of the air above the specimen, and the microprocessor set this value as the null, or reference point for the measurement.

Step 2

Next, the thermocouple is Peltier-cooled below the dew point temperature. Microscopic water droplets begin to condense upon the thermocouple surface.

Step 3
The microprocessor then allows the thermocouple temperature to be controlled exclusively by the water condensing upon it. The heat of the condensation causes the thermocouple temperature to rise, converging upon the exact temperature at which condensation ceases. This stable condition is achieved approximately one minute after sample insertion.

Step 4
The point of temperature stability is the dew point temperature. The final readout on the instrument display is proportional to the dew point temperature depression with a resolution of 0.0003 °C. Because dew point temperature depression is an explicit function of vapor pressure, the instrument can be calibrated to report results directly in Standard International units of osmolality, mmol/kg.


Hemotology Slide Stainer Cytocentrifuge Specifications

Sample Volume 10 µL nominal (Larger samples or samples as small as 2 µL can be measured reliably with special procedures)
Measurement Range Typically 0 to 3200 mmol/kg @ 25° C ambient (up to 3800 mmol/kg with extended range option)
Measurement Time 75 seconds
Resolution 1 mmol/kg
Repeatability Linearity Readout Standard deviation = 2 mmol/kg, 2%of reading from 100 to 2000 mmol/kg, 10 X 6.8 cm LCD

Operating Temperature

15 to 37° C ambient (instrument should be at stable temperature before calibrating)
Calibration Automatic using Opti-mole™ osmolality standards (non-battery memory backup system)
Serial Output RS-232 (ASCII format)
Electrical
Line Voltage:  100 to 120 V or 220 to 240 V nominal, 50 to 60 Hz (set at factory, user-selectable with fuse change)
Power: Less than 5 watts
Fuses: 1/8 amp time-delay type for 100/120 volts (2 required) - 1/16 amp time-delay type for 220/240 volts (2 required)
Height 17 cm (6.6”)
Width 29 cm (11.5”)
Depth 34 cm (13.5”)
Weight 3.6 kg (8 lbs)
Buyer's Specifications

The osmometer shall be a Wescor Model 5520 Vapor Pressure Osmometer and shall operate on the vapor pressure depression principle. The osmometer shall be capable of routine osmolality determinations on sample volumes of 2-10 microliters and shall perform the measurement automatically once the sample has been loaded. The osmometer shall be capable of routine osmolality determinations. The osmometer shall consume no more than 30 watts of electrical power and shall weigh less than 4 kg.


Ordering Information Hematology Slide Stainer Cytocentrifuge
Cat. No. Item
W-5520 Vapro Vapor Pressure Osmometer with AC-066 Head for 0-3200 mmol/kg range
Base Price includes the following
;
  • OA-010 Optimol Osmolality Ampoule Standard, 100 mmol/kg (1 package)
  • OA-029 Optimol Osmolality Ampoule Standard, 290 mmol/kg (1 package)
  • OA-100 Optimol Osmolality Ampoule Standard, 1000 mmol/kg (1 package)
  • AC-061 Ampoule Organizer for Osmolality Standards and Control(1 each)
  • SS-003 Cleaning Solution, for manual thermocouple cleaning (1 bottle)
  • SS-006 Deionized Water (60 mL dropper bottle) (1 bottle)
  • SS-033 Sample Discs (vial of 5000) (1 vial)
  • SS-036 Micropipettor Disposable Tips for AC-024 and AC-037 (1 package)
  • AC-037 Micropipettor, 10 UL (1 each)
  • OM-275 Hex Driver, 9/64 inch, screwdriver handle (1 each)
  • OM-300 Forceps, 5 inch, stainless steel (1 each)
  • V-1003 or V-1001 Thermocouple Cleaning Video (1 each)
  • Illustrated User's Manual
  • One-Year Parts & Labour Warranty
5520XR Vapro Vapor Pressure Osmometer with AC-067 Head for above 3200 mmol/kg range
Base price includes the same accessories as the W-5520 listed above.
Accessories & Supplies
AC-037 Micropipettor, 10 UL
AC-061 Ampoule Organizer for Osmolality Standards and Control
RP-076 Thermocouple Head Assembly , model 5500 REBUILT - this item must be exchanged with original head assembly - otherwise an additional fee will be charged
RP-173 Thermocouple Head Assembly , model 5520 REBUILT - this item must be exchanged with original head assembly - otherwise an additional fee will be charged
OM-275 Hex Driver, 9/64 inch, screwdriver handle
OM-300 Forceps, 5 inch, stainless steel
121275 Vapro Printer Cable
AC-062 Standard Sample Holder, 7 mm x 1.25 mm deep, models 5520, 5500, 5100C
Special Purpose Sample Holders for small and gross samples
AC-063 Sample Holder for 2 UL Samples, 4.25 mm dia. x 1.12 mm deep, models 5520, 5500, 5100C
AC-064 Sample Holder, 7 mm dia. x 2.5 mm deep, models 5520, 5500, 5100C
AC-065 Sample Holder, 9.5 mm dia. x 4.5 mm deep, models 5520, 5500, 5100C
AC-078 Sample Holder for Kwikdisk 17-0643 O-RING for Standard Sample Holder
Osmometry Standards/Controls
Optimol Osmolality Ampoule Standards, 0.4 mL Vial (package of 60)
OA-010
OA-029
OA-100
Optimol Osmolality Ampoule Standard, 100 mmol/kg
Optimol Osmolality Ampoule Standard, 290 mmol/kg
Optimol Osmolality Ampoule Standard, 1000 mmol/kg
SS-039 Osmolality Control, 1 mL vial (package of 6) 2 high, 2 normal, 2 low
  • High is approx 330 mmol/kg - lot specific
  • Normal is approx 290 mmol/kg - lot specific
  • Low is approx 270 mmol/kg - lot specific
SS-003 Cleaning Solution, for manual thermocouple cleaning (60 mL dropper bottle)
SS-004 No longer available
SS-006 Deionized Water (60 mL dropper bottle)
SS-026 Blow Clean
SS-033 Sample Discs (vial of 5000)
SS-036 Micropipettor Disposable Tips for AC-024 and AC-037 (package of 1000)
Manuals & Instructional Materials
M2448 Vapor Pressure Osmometer User's Manual, model 5500, 5500XR
M2468 Vapro Vapor Pressure Osmometer User's Manual, model 5520
M2469 Vapro Vapor Pressure Osmometer Service Manual, model 5520
V-1001 Thermocouple Cleaning Video, model 5500, VHS Format (specify NTSC, PAL, or SECAM)
V-1003 Thermocouple Cleaning Video, model 5520, VHS Format (specify NTSC, PAL, or SECAM)


Canadian Customers if you wish to speak to a sales representative or customer service, please call Toll Free within Canada: 1-888-883-9101. Outside Canada, click here for ordering information.

Vapro Vapor Pressure Osmometer Downloadable Brochure:
  5520-Vapro Vapour Pressure Osmometer - PDF Format (442 kb)

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Vapro® Vapor Pressure Osmometer - Discovery Diagnostics, Canadian distributor for Wescor's Vapor Pressure Osmometer for measurement of whole blood, viscous samples & tissue specimens